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Team Project Name neurons in the brain at the single-cell resolution, so as
to unravel the mystery of the structural and functional
Record Voxel Rate Nonlinear Optical Microscope to aspects of brain connectome
Unravel Brain Connectome and Signaling 6.To answer the fundamental questions of critical neuro-
Research Project diseases.

Identifying and unraveling the mystery of the structural 7.To establish international collaboration linkage with
National Institute of Health, USA.
and functional aspects of
In a nut-shell, this program will not only realize record-
brain connectome will usher a new paradigm shift in performance microscopy platforms for brain studies,
the present research and clinical practice. With the but also usher new horizon in the development of
new end point of Taiwan Brain Innovative Technology therapeutics for neurodegenerative diseases as well as
Development and Application (Brain-ICT) Program in cancers.
mind, in this continued research proposal, we not only
aim to develop a disruptive rapid fresh pathological Key Words
platform for intraoperational brain tumor assessment,
but also aim to fulfill our original objective, which is to Neuronal circuits, connectome, high resolution
develop innovative and interruptive optical imaging mesoscope, voltage imaging, kilohertz microscopy,
platforms with a record data throughput and voxel rate microendoscope, AADC deficiency, suprachiasmatic
so as to construct a function connectome of different nucleus, circadian rhythm, brain tumor, intraoperational
neurons in the brain at the single-cell resolution, to tumor assessment, rapid fresh pathology, artificial
decipher the details of the brain connectome, and to intelligence, deep learning
answer the fundamental questions of critical neuro-
diseases, with the following two scientific aims: first, Global Cooperation
to answer the question why Aromatic L-amino acid
decarboxylase deficiency (AADC deficiency) subjects still Our team have currently completed the deep brain
have cyclic oculogyric crisis (OGC) after gene therapy, functional calcium imaging system and is capable of
and second, to understand the complete firing pattern recording at a speed of 23 Hz using two-photon laser
of suprachiasmatic nucleus (SCN) in hypothalamus and scanning system. Our two photon system is different from
how coupling is formed between neurons in the SCN. the single-photon miniature imaging systems offered
In short, in this proposal, we will extend the previous by manufacturers, which utilizes only single photon
brain technology breakthrough to further incorporate the microscopy for recording. Our two photon system results
strength of Taiwan’s ICT superiority and to continuously in higher resolution and is not affected by out-of-focus
develop innovative and interruptive optical brain neural signals of the observed field of view. Additionally,
imaging platforms so as to provide imaging solutions for it allows for 3D recording and the possibility of recording
clinical brain surgent, for drug discovery institutes, and approximately 100 um thick tissues in separate sessions,
for academic brain scientists. The scientific program which is not achievable with typical single-photon
endpoints include: miniature imaging systems. As a result, research teams
interested in conducting in vivo functional recordings of
1.To further advance the international competitiveness neural activity in deep brain regions such as the mouse
and to continuously develop the record voxel-rate suprachiasmatic nucleus (SCN) of the central circadian
multiphoton 3D mesoscope for potential commercial clock and the substantia nigra compacta (SNc) where
usage. dopamine neurons are located can collaborate with us.
2.To further incorporate electronics engineering so as to In addition, scientific research topics related to circadian
rhythm regulation, motor control, and neurodegenerative
leverage Taiwan’s ICT superiority diseases are our specific topic for potential collaboration.
3.To technology transfer to and to collaborate with
domestic companies to leverage Taiwan’s Brain Project Web Page
technology industry.
4.To provide solution toward unmet clinical needs for
deadly brain diseases.
5.To construct a function connectome of different
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